I have determined that a friend of mine, who works in another lab, has access to materials I could use for inflating caterpillars. I can’t wait until the collecting season starts and I can start on my own specimens.
While some inflated caterpillars are curated within the main collection, and some of them are grouped by family in the larval cabinets, there are many “miscellaneous” drawers with arrangements like this.
All sorts of caterpillars! The nice green color of the swallowtail caterpillars (bottom right-ish) was not preserved though. This seems to be one limitation of caterpillar inflation – patterns remain, but colors become lost or distorted. Most inflated caterpillars are some shade of tan/brown. This could be due to the heating process or simply by fading over time due to light exposure.
One thing I did not expect to see – everted osmeterium! Swallowtail caterpillars (Papilio) have an eversible gland called the osmeterium which is normally held inside the body. When threatened, the caterpillar sticks out the two forks and tries to touch the attacker – wiping them with smelly defensive chemicals. Just the smell could be enough to deter a would-be predator, but I’m sure the taste is pretty bad too.
Many of the geometrid caterpillars were preserved in their usual hunched position. I wonder if this was induced somehow during the heating process, or if they simply inflated into the most natural position?
I couldn’t help but include a photo of the megalopygids (fuzzy guys on the right). Preserving them must have been a delicate task, hopefully carried out with gloves – they can give quite a vicious sting! They look more like little mice than insects, and each hair can leave a welt on your skin. I was tempted to test whether old preserved specimens would still sting, but decided not to try.
Have any of you ever inflated a caterpillar specimen before? If yes, do you have any advice?
There are several ways to preserve caterpillars for study. You can boil them and drop them into a jar of alcohol, you can freeze dry them, or you can inflate them.
The photos in this post are all of inflated caterpillars, in the genus Acronicta, found at the Cornell University Insect Collection (CUIC).
Inflating (or “blowing”) caterpillars is a traditional technique that is no longer taught or commonly used, however it can produce fabulous results. The caterpillar remains dry and can be kept in a drawer along with adults, which makes them easier to study than specimens in alcohol. Their external features are usually well maintained – specimens from the early 1900s don’t look much different from modern specimens.
However they are lacking their internal organs, and the last couple of segments are destroyed in the preparation process. The technique is also quite labor intensive and requires unusual equipment. So how does it work?
I have talked to a few entomologists who have inflated caterpillars, but not since their undergraduate or graduate school days. I have found a few resources which describe the process. Here is a description from the University of Minnesota Agricultural Experiment Station:
Basically you cut a slit into the posterior end of the caterpillar, and using a pencil, roll out the guts (starting at the head end). You must be careful to not destroy the hairs and other features of the exterior. Then you insert a glass tube and inflate the caterpillar, and keep the caterpillar inflated as you heat it in a small make-shift oven. One colleague described using a coffee can over a bunsen burner for this purpose.
Once the caterpillar is dry, the glass tube is removed and the caterpillar can be prepared for pinning. Either a wire or stick can be inserted, or the caterpillar can be glued onto the wire or stick by its prolegs underneath the body.
A box of Acronicta americana adults, caterpillars, and pupae
Late (above) and early (below) instars of Acronicta morula
Another benefit of inflating caterpillars is that the body shapes are extended and not distorted by being kept in a liquid. The important bumps and hair positions are kept as their were in life. However the body may be a bit overextended depending on how well it was squished and inflated.
One reason I particularly enjoy the inflated caterpillars is that they are easy to transport and trade – so there are many European species in collections here in the USA. Caterpillars are not often kept in collections to begin with, so to have these specimens available to me for study is quite exciting.
As you can probably guess, I’m going to set up my very own caterpillar inflating oven as soon as I can! And I’ll have another post soon on even more inflated caterpillars I have found at other collections.
University of Minnesota, 1908. 16th Annual Report of the Agricultural Experiment Station. Bulletin #105. How to Collect and Preserve Insects. Pages 119-146.